Mighty Leo Mastermix is 2X concentrated solution for PCR reaction which contains dNTPs, Taq DNA Polymerase, Mg+2 and other critical reaction components. It doesn’t contain primers and DNA template. Leo Mastermix is designed for routine PCR and also for amplification of high GC templates. The pre loaded green dye makes it easier for users to load directly on the agarose gel post PCR.
- Extremely powerful Taq based premix, specially optimized for GC rich templates.
- 5X better yield than any standard Taq DNA polymerase.
- Amplification upto 5KB is tested.
- Comes with pre-loaded green dye which separates into blue (Equivalent to ~ 3-5kb DNA fragment) and yellow (Equivalent to ~25bp DNA fragment) on 1% agarose gel.
- Super thermostable: Mastermix is made with proprietary formulation buffer which makes shipping possible at Room Temperature. The mastermix is tested for its massive amplification, even after incubating at 45℃ for 7 days !
The mastermix contains Taq DNA Polymerase whose gene was cloned from Thermus aquaticus and expressed in E.Coli.
- For long time storage, the products can be stored at -20℃.
- For routine/daily usage, the products can be stored at 4℃ for upto 6 months.
- Avoid primer contamination and protect from nucleases.